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NHERF-1 and NHERF-2 share sequence homology, and both associate with β-catenin. (A) Schematic diagram of NHERF-1 and NHERF-2. Highlighted are the conserved domains and sequence identities. (B) NHERF coimmunoprecipitates with β-catenin. NHERF-1 and NHERF-2 were immunoprecipitated from total HMEC-1 cell lysate. The immunoprecipitates were resolved by SDS-PAGE and immunoblotted for β-catenin. Lane 1 shows the amount of β-catenin in total cell lysate.
