Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Apr;18(4):1220–1232. doi: 10.1091/mbc.E06-10-0960

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 by The American Society for Cell Biology

PMC Copyright notice

Figure 7. — β-Catenin and N-cadherin form a complex with PDGF-Rβ and NHERF-2 in HT1080 cells. (A) HT1080 cells expressing NHERF-2 (a and b) or the first PDZ domain of NHERF-2 (c and d) were fixed on coverslips and costained for β-catenin (rabbit) and NHERF-2 using 33E2 against PDZ I. Deconvolved images are shown. (B) The C terminus of PDGF-Rβ fused to MBP or MBP alone were immobilized on amylose and incubated with SW707 extract in the presence of GST-wild type NHERF-2 (GST-N2) or GST-PDZ I domain of NHERF-2 (PDZ I). The reactions were resolved by SDS-PAGE and immunoblotted for β-catenin (top) or GST (bottom). (C) Extracts of HT1080 cells expressing PDGF-Rβ and full-length NHERF-2 (lanes 1, 2, and 4) or the first PDZ domain of NHERF-2 (lane 3) were immunoprecipitated with control antibody (lane 2) or anti-PDGF receptor (lanes 3 and 4), resolved by SDS-PAGE and immunoblotted for β-catenin. (D) Extracts of HT1080 cells expressing PDGF-Rβ and full-length NHERF-2 (lanes 1, 2, 5, and 6) or the first PDZ domain of NHERF-2 (lanes 3 and 4) were immunoprecipitated with control antibody (lane 2), antibodies against N-cadherin (lanes 4 and 6), or antibodies against β-catenin (lanes 3 and 5) and immunoblotted for PDGF-Rβ.