Skip to main content
. 2007 Apr;18(4):1220–1232. doi: 10.1091/mbc.E06-10-0960

Figure 9.

Figure 9.

The N-cadherin/β-catenin/NHERF/PDGF-R complex contributes to HT1080 cell migration. (A) Confluent monolayers of parental HT1080 cells, HT1080 cells expressing exogenous NHERF-2, (HT + NHERF-2), HT1080 cells expressing the PDZ I domain of NHERF-2 that cannot bind to β-catenin (HT + PDZ I), HT1080 cells expressing shRNA against N-cadherin (HT + N-cad KD), HT1080 cells expressing shRNA against endogenous NHERF1 (HT + NHERF-1 KD), HT1080 cells expressing the β-catenin mutant unable to bind to NHERF (HT + β-cat DT-AA) or parental HT1080 cells treated with PDGF-R inhibitor (HT + PDGF-R inhibitor) were scratched with a sterile pipette tip and allowed to migrate into the wound for 10 h. (B) The above-mentioned assays were quantified by measuring the amount of denuded dish remaining at the specified times. Three independent assays were done and the SD calculated for the final time point (10 h).