Figure 1.

Actin oxidation in oyeΔ cells leads to ROS accumulation. Logarithmically growing cells were stained for 3.5 h with the ROS-sensing dye H₂DCFDA, and the percentages of fluorescent cells was determined. □, rho⁺ cells; ▩, rho⁰ cells. The averages and SDs reported are for three to four experiments. Left inset, example of an ROS-negative wild-type cell; right inset, example of an ROS-positive oyeΔ cell.