Figure 5.

N-acetyl-l-cysteine (NAC) suppresses the actin organization and accelerated cell death phenotype of oye2Δ cells. (A) NAC was added to logarithmically growing cultures at a final concentration of 31 mM for 5.5 h, after which the cells were fixed, stained with rhodamine phalloidin and visualized by fluorescence microscopy. (B) NAC was added at a final concentration of 31 mM to stationary cultures on days 4 or 6 (indicated by arrows). Cells were removed at the indicated time points, dilutions were plated, and percent viability was calculated based on colony-forming units per total cell density. The arrows with dotted lines show the increases in viability at day 5 for NAC addition at day 4 and at day 8 for NAC addition at days 4 and 6. (C) The box highlights a portion of Figure 4B, with only the oye2Δ samples highlighted at days 2, 5, and 8.