Figure 4.

Effect of Prox1 on the migration of HUVECs stimulated with VEGF-A and VEGF-C. Cell migration was measured by Boyden chamber assay as described in Materials and Methods. HUVECs were infected with adenoviruses (Ad) encoding DNA-binding mutant (Mut) or wild-type (WT) Prox1 in combination with those encoding wild-type (WT), dominant-negative mutant form (d.n. Mut) of VEGFR3, or Null (encoding no transcripts) and plated on Boyden chambers with indicated chemoattractants placed in lower wells. Ad-Null was used in order to infect HUVECs with the same quantities of adenoviruses for all of samples. Results are expressed as the ratio of number of migrated cells normalized to control (no attractant). Each value represents the mean of triplicate determinations; bars, SD.