Figure 5.

Forskolin treatment causes lysosome acidification and degradation of Cy3f-Aβ by microglia. (A) Lysosomal pH was measured 45 min after addition of 200 μM forskolin. Where indicated, 1 mM DIDS was added 15 min before forskolin addition. Lysosomal pH measurements were obtained from at least 750 lysosomes from four different fields. The average pH values from four different experiments from four different days are shown. Error bars represent the SEM. (B) Microglia were incubated for 60 min with 7 μg/ml Cy3-fAβ and chased for 72 h. During the chase period, the cells were treated with 45-min pulses of 200 μM forskolin to activate PKA during the 1st, 6th, 24th, and 48th h. Cy3 fluorescence power after 72 h normalized to the fluorescence power at the 0-h time point. The data shown here represent average of the normalized intensity values from four different experiments done on four different days. Error bars represent the SEM.