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. 2007 Apr;18(4):1490–1496. doi: 10.1091/mbc.E06-10-0975

Figure 6.

Figure 6.

Lysosomal pH measurements in activated microglia in presence of PKA inhibitor and chloride channel blocker. Lysosomal pH was measured in treated and untreated microglia. For measurements with PKA inhibitor, KT 5720 at 10 μM was added to MCSF-treated microglia, and pH was measured 45 min after addition. For measurements in the presence of the chloride channel blocker DIDS at 1 mM was added to MCSF-treated microglia, and lysosomal pH was measured 60 min after the addition of DIDS. (A) Frequency distribution of lysosomal pH in MCSF-treated microglia (green curve), untreated microglia (red curve), and KT 5720-treated activated microglia (blue curve). (B) Frequency distribution of lysosomal pH in MCSF-treated activated microglia (green curve), untreated microglia (red curve), and DIDS-treated activated microglia (black curve). Lysosomal pH measurements were obtained from at least 750 lysosomes from three different fields. The average pH values from three different experiments from three different days are shown. Error bars represent the SEM.