Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Apr;18(4):1457–1463. doi: 10.1091/mbc.E06-10-0908

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 by The American Society for Cell Biology

PMC Copyright notice

Figure 6. — Regulation of Skp2 and p27 by cyclin D1 RNAi. MEFs transfected with control (C) or cyclin D1 siRNA were serum-starved and then replated at subconfluence with 10% FBS for 20 h. (A) A portion of the collected cells were analyzed by QPCR for Skp2 mRNA and 18S rRNA; the level of Skp2 mRNA plotted relative to 18S rRNA is shown. (B) The remainder of the cells was analyzed by Western blotting for Skp2, cyclin D1, and actin (loading control). (C) Collected cell lysates from an independent experiment were Western blotted for p27, cyclin D1, and actin.