Figure 1.

Interaction between ATXN2 and the DEAD/H-box RNA helicase DDX6. (A) Schematic illustration of ATXN2. Ellipses represent the polyQ-region (dark red), LSm and LSmAD domain (dark blue and light blue, respectively). Bars below display ATXN2 regions used in the directed yeast two-hybrid analysis. (B) Yeast two-hybrid analysis. Yeast strain L40ccua was transformed with the respective plasmids to coexpress the different LexA and AD fusion proteins as indicated. Afterward, transformants were isolated and spotted on selective media or on a membrane to analyze the activity of the reporter genes. (C) Coimmunoprecipitation. Cell lysates were prepared from HEK293T and SH-SY5Y cells. Coimmunoprecipitation was performed with 5 μl of α-ATXN2 antibody (left) or 1 μl of α-DDX6 antibody (right). Precipitated proteins were visualized with the antibodies indicated.