Figure 4.

The LSm/LSmAD domain of ATXN2 is accountable for the interference with P-body structures. SH-SY5Y cells were transiently transfected with plasmid pCMV-MYC-ATXN2-LSm/LSmAD or pTL-FLAG-ATXN2-LSm/LSmAD. For staining exogenous ATXN2 and endogenous DDX6, cells were incubated with antibodies directed against the MYC- or FLAG-tag and DDX6, followed by treatment with secondary antibodies coupled to FITC or Cy3, respectively. To overexpress an unrelated protein as control, cells were transfected with plasmid pTL-FLAG-endophilin-A3. Nuclei were stained with Hoechst. Bars, 10 μm. Arrows indicate transfected cells.