Figure 6.

A reduced ATXN2 level prevents SG assembly. Top, HEK293T cells were cotransfected with a mix of siATXN2#2 and siATXN2#3 molecules. As controls, siControl#1 and siControl#2 were used as indicated. Sixty-eight hours post transfection, cells were incubated with 0.5 mM arsenite for 1 h. Nuclei were stained with Hoechst. Bars, 10 μm. Bottom, quantitative analysis. The percentage of TIA-1–positive SGs in control cells versus knockdown cells was determined using the AxioVision software. The number of cellular SGs was counted in each picture taken and divided through the cell number. The mean value of SGs in the cells counted was calculated and SD was weighted. Finally, the number of SGs counted for the control cells was set as 100%, and the number of SGs counted for the knockdown cells was aligned.