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. 2007 Apr;18(4):1282–1292. doi: 10.1091/mbc.E06-09-0846

Figure 6.

Figure 6.

CF Im colocalizes with nascent transcripts. (A) RNA polymerase II (small grains) colocalizes with CFIm (large grains) on some PFs (arrowhead). Inset I, at higher magnification, the double labeling is shown on a single, nascent PF. Bar, 0.1 μm. (B) Newly incorporated BrU (small grains) colocalizes with CFIm (large grains) on PFs (arrowhead). Inset, a single, double-labeled PF. Nc, nucleolus. Inset II, a single PF, labeled with anti-BrU antibody (6-nm gold), anti-CF Im68 antiserum (12-nm gold), and anti-CstF antibody (18-nm gold). Bar, 0.1 μm. (C) Newly incorporated BrU (small grains) colocalizes with CFIm (large grains) on PFs (arrowhead). Inset, a single, double-labeled PF. Nc, nucleolus. Bar, 0.1 μm. (D) Immunolabeling for CFIm after selective staining of RNA with terbium. Inset, double immunolabeling for CFIm (large grains) and SC35 (small grains) of terbium-stained RNA. The arrowhead indicates labeling on PFs, whereas interchromatin granules (ig) are unlabeled. Bar, 0.1 μm. (E) HeLa cells treated with DRB. The labeling is decreased on PFs, but still intense on IGAZs (large arrow). Inset, another IGAZ labeled for CFIm after DRB treatment. Ig, interchromatin granules; nc, nucleolus. Bar, 0.1 μm.