The domain of histone H4 hypoacetylation at HML. (A) Relative fraction of different sequences within and adjacent to HML immunoprecipitated with anti-acetylated histone H4 Ab. See text for calculations. (B) SIR-dependent relative acetylation of histone H4 as a function of position on chromosome III. (Upper) Schematic representation of the 11- to 200-kb sequence of chromosome III analyzed in the chromatin immunoprecipitation assay. The HMLα and MATa loci and the CHA1 gene are indicated. Various DNA sequences (coordinates in parentheses) used to make probes for slot blot analysis are: a (13172–13418), b (14839–15360), c (15730–16264), d (16264–16714), e (16714–17562), f (17562–18049), g (19574–20029), and h, the MATa-specific probe (16). (Lower) SIR-dependent relative acetylation of histone H4 described in A, plotted as the function of the positions of the a–h probes.