Fig. 3.

Knockdown of POMC expression results in the decrease of inhibitory effect of CRH on NF-κB signaling. Cells were transfected with POMC antisense or scrambled oligonucleotides, pre-incubated in Epilife medium with EDGS for 24 h and then incubated in Ham’s F10 medium without growth factors for 24 h without or with 100 nM CRH. NF-κB DNA binding activity was estimated by p65 activity ELISA. Inset: cells were transfected with NF-κB construct and POMC antisense or scrambled oligonucleotide, pre-incubated in Epilife medium with EDGS for 24 h and then incubated in Ham’s F10 medium without growth factors for 24 h with or without 100 nM CRH. NF-κB transcriptional activity was estimated by the luciferase signal. The data is presented as mean ± S.E.M. (n = 3) in comparison to the cells not treated with CRH (=100%). ^*P < 0.05 control vs. CRH, ^#P < 0.05 cells treated with CRH and transfected with POMC antisense oligonucleotides vs. transfected with scrambled antisense oligonucleotides.