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. Author manuscript; available in PMC: 2007 Mar 29.
Published in final edited form as: Mol Microbiol. 2005 May;56(4):1017–1034. doi: 10.1111/j.1365-2958.2005.04566.x

Table 3.

Spearman rank correlation coefficient (r) between cognate amino acid bias of the amino acid biosynthetic enzymes and their molecular activity.

E. coli S. typhimurium B. subtilis Overall
r (n = 79)d,e r (n = 79)d,e r (n = 89)d,e r (n = 247)d,e
Molecular activitya 0.284 (P < 0.0033) 0.301 (P < 0.0030) 0.186 (P < 0.010) 0.230 (P < 5 × 10-7)
Protein costb -0.173 (P < 0.05) -0.104 (P < 0.15) 0.147 (P < 0.04) -0.0819 (P < 0.05)
GC contentc 0.216 (P < 0.02) 0.154 (P < 0.07) 0.157 (P < 0.04) 0.164 (P < 0.01)
Codon biasc -0.201 (P < 0.03) 0.0975 (P < 0.18) 0.0580 (P < 0.27) -0.0322 (P < 0.24)
a

Calculated as described in Experimental procedures.

b

Calculated by adding the costs for synthesizing each of the amino acid residues that constitute the enzymes of a given amino acid biosynthetic pathway.

c

Calculated for the genes that encode the enzymes of a given amino acid biosynthetic pathway.

d

n represents the number of enzyme activities used to calculate the correlations. It can be determined from Table 2.

e

P represents the probability that the correlation is non-significant (see Experimental procedures).

Also shown for comparison are correlations between cognate amino acid bias of the amino acid biosynthetic enzymes and three other factors that could potentially influence the amino acid composition of the same biosynthetic enzymes. These three factors have been previously identified as influencing amino acid composition of the total proteome