Table 4.
Significance of the Spearman rank correlation coefficient (r) between amino acid bias and molecular activity of enzymes involved in amino acid biosynthesis.a
| E. coli | S. typhimurium | B. subtilis | Overall | |
|---|---|---|---|---|
| r (n = 79)b,c | r (n = 79)b,c | r (n = 89)b,c | r (n = 247)b,c | |
| Ala | -0.0299 (P < 0.38) | -0.0390 (P < 0.35) | 0.118 (P < 0.084) | -0.0489 (P < 0.16) |
| Arg | -0.00708 (P < 0.47) | -0.0599 (P < 0.28) | 0.00803 (P < 0.47) | -0.0883 (P < 0.038) |
| Asn | -0.0869 (P < 0.19) | -0.0708 (P < 0.24) | 0.160 (P < 0.032) | -0.0121 (P < 0.40) |
| Asp | -0.127 (P < 0.10) | -0.157 (P < 0.058) | -0.332 (P < 4.8 × 10-5) | -0.155 (P < 0.00045) |
| Cys | 0.0288 (P < 0.41) | -0.0334 (P < 0.37) | 0.0420 (P < 0.36) | -0.00400 (P < 0.46) |
| Gln | 0.171 (P < 0.054) | 0.0300 (P < 0.39) | -0.0316 (P < 0.36) | 0.103 (P < 0.020) |
| Glu | -0.175 (P < 0.043) | 0.0159 (P < 0.43) | -0.0641 (P < 0.22) | -0.0392 (P < 0.21) |
| Gly | -0.0731 (P < 0.23) | 0.156 (P < 0.061) | 0.0145 (P < 0.44) | 0.0372 (P < 0.23) |
| His | -0.0178 (P < 0.41) | -0.176 (P < 0.041) | -0.0218 (P < 0.39) | -0.129 (P < 0.0046) |
| Ile | 0.103 (P < 0.17) | 0.108 (P < 0.14) | 0.241 (P < 0.0024) | 0.0827 (P < 0.049) |
| Leu | -0.0469 (P < 0.31) | -0.107 (P < 0.14) | -0.106 (P < 0.11) | -0.0806 (P < 0.052) |
| Lys | -0.0802 (P < 0.21) | 0.0549 (P < 0.30) | -0.0912 (P < 0.14) | 0.0337 (P < 0.25) |
| Met | 0.259 (P < 0.0067) | 0.289 (P < 0.0022) | -0.0801 (P < 0.17) | 0.234 (P < 1.3 × 10-6) |
| Phe | -0.00113 (P < 0.13) | -0.168 (P < 0.048) | -0.231 (P < 0.0033) | 0.102 (P < 0.020) |
| Pro | -0.134 (P < 0.091) | -0.0370 (P < 0.35) | 0.154 (P < 0.036) | -0.0730 (P < 0.069) |
| Ser | 0.130 (P < 0.1) | 0.197 (P < 0.025) | 0.0592 (P < 0.25) | 0.128 (P < 0.0050) |
| Thr | 0.183 (P < 0.043) | 0.131 (P < 0.10) | -0.0357 (P < 0.34) | 0.0667 (P < 0.093) |
| Trp | -0.107 (P < 0.11) | -0.176 (P < 0.028) | -0.174 (P < 0.0033) | -0.111 (P < 0.0066) |
| Tyr | -0.176 (P < 0.042) | -0.131 (P < 0.094) | 0.130 (P < 0.064) | -0.0598 (P < 0.10) |
| Val | 0.179 (P < 0.045) | 0.0552 (P < 0.29) | -0.0177 (P < 0.42) | 0.0755 (P < 0.065) |
| CBd | 0.284 (P < 0.0033) | 0.301 (P < 0.0030) | 0.186 (P < 0.01) | 0.230 (P < 5 × 10-7) |
a
Values in bold are those that are significant to a level higher than 99% and r > 0.20.
b
n represents the number of enzyme activities used to calculate the correlations. It can be determined from Table 2.
c
P represents the probability that the correlation is non-significant (see Experimental procedures).
d
CB indicates cognate amino acid bias.