Figure 7.
Low dose of GM-CSF influences microglial CD40 expression in response to S. aureus. Primary microglia expanded either with (+) or without (-) GM-CSF were seeded into 6-well plates at 2 × 106 cells per well and incubated overnight. The following day, cells were either unstimulated or treated with heat-inactivated S. aureus (107 cfu/well) for 24 h, whereupon microglia were recovered and stained with MHC class II, CD40, CD80, or CD86 antibodies and subsequently with a FITC-conjugated secondary antibody for flow cytometric analysis. Microglia were stained with an isotype-matched control antibody to assess background staining. Results are representative of two independent experiments.