Table 2.
Gene Ontology annotation
| Solenopsis invicta EST library | D. melanogaster genome | ||||||
| Blastx-determined GO | Prosite-determined GO | ||||||
| Molecular function | 4,301* | (100.0%) | 486* | (100.0%) | 14,778* (100.0%) | ||
| Antioxidant activity | 20 | (0.5%) | 2 | (0.4%) | 39 (0.3%) | ||
| Binding | 1,765 | ↑ | (41.0%) | 174 | (35.8%) | 4,319 (29.2%) | |
| Catalytic activity | 1,456 | ↑ | (33.9%) | 201 | ↑ | (41.4%) | 4,072 (27.6%) |
| Chaperone regulator activity | 5 | ↑ | (0.1%) | 0 | (0.0%) | 1 (0.0%) | |
| Enzyme regulator activity | 91 | (2.1%) | 7 | (1.4%) | 382 (2.6%) | ||
| Molecular function unknown | 145 | ↓ | (3.4%) | 6 | ↓ | (1.2%) | 1,852 (12.5%) |
| Motor activity | 29 | (0.7%) | 1 | (0.2%) | 88 (0.6%) | ||
| Nutrient reservoir activity | 14 | ↑ | (0.3%) | 0 | (0.0%) | 8 (0.1%) | |
| Obsolete molecular function | 0 | (0.0%) | 9 | ↑ | (1.9%) | 0 (0.0%) | |
| Signal transducer activity | 153 | ↓ | (3.6%) | 4 | ↓ | (0.8%) | 1,091 (7.4%) |
| Structural molecule activity | 210 | (4.9%) | 59 | (12.1%) | 759 (5.1%) | ||
| Transcription regulator activity | 116 | ↓ | (2.7%) | 4 | (0.8%) | 841 (5.7%) | |
| Translation regulator activity | 62 | ↑ | (1.4%) | 7 | (1.4%) | 92 (0.6%) | |
| Transporter activity | 235 | (5.5%) | 12 | (2.5%) | 1,014 (6.9%) | ||
| Triplet codon-amino acid adaptor activity | 0 | ↓ | (0.0%) | 0 | (0.0%) | 220 (1.5%) | |
| Cellular component | 4,838* | (100.0%) | 362* | (100.0%) | 14,986* (100.0%) | ||
| Cell† | 1,868 | ↑ | (38.6%) | 147 | (40.6%) | 5,225 (34.9%) | |
| Cellular component unknown | 85 | ↓ | (1.8%) | 0 | ↓ | (0.0%) | 1,920 (12.8%) |
| Envelope | 107 | (2.2%) | 1 | (0.3%) | 290 (1.9%) | ||
| Extracellular matrix | 14 | (0.3%) | 0 | (0.0%) | 46 (0.3%) | ||
| Extracellular matrix part | 4 | (0.1%) | 0 | (0.0%) | 23 (0.2%) | ||
| Extracellular region | 73 | ↓ | (1.5%) | 2 | (0.6%) | 416 (2.8%) | |
| Extracellular region part | 23 | (0.5%) | 0 | (0.0%) | 88 (0.6%) | ||
| Membrane-enclosed lumen | 160 | (3.3%) | 3 | (0.8%) | 515 (3.4%) | ||
| Organelle | 1,360 | ↑ | (28.1%) | 100 | (27.6%) | 3,007 (20.1%) | |
| Organelle part | 548 | (11.3%) | 22 | (6.1%) | 1,632 (10.9%) | ||
| Protein complex | 575 | (11.9%) | 87 | ↑ | (24.0%) | 1,756 (11.7%) | |
| Synapse | 7 | (0.1%) | 0 | (0.0%) | 40 (0.3%) | ||
| Synapse part | 3 | (0.1%) | 0 | (0.0%) | 27 (0.2%) | ||
| Virion† | 11 | ↑ | (0.2%) | 0 | (0.0%) | 1 (0.0%) | |
| Biological process | 5,453* | (100.0%) | 630* | (100.0%) | 22,798* (100.0%) | ||
| Biological process unknown | 61 | ↓ | (1.1%) | 0 | ↓ | (0.0%) | 888 (3.9%) |
| Cellular process | 2,242 | ↑ | (41.1%) | 297 | ↑ | (47.1%) | 7,772 (34.1%) |
| Development | 121 | ↓ | (2.2%) | 0 | ↓ | (0.0%) | 2,148 (9.4%) |
| Growth | 17 | (0.3%) | 0 | (0.0%) | 102 (0.4%) | ||
| Interaction between organisms | 6 | (0.1%) | 0 | (0.0%) | 92 (0.4%) | ||
| Physiological process | 2,328 | ↑ | (42.7%) | 315 | ↑ | (50.0%) | 7,858 (34.5%) |
| Pigmentation | 1 | (0.0%) | 0 | (0.0%) | 51 (0.2%) | ||
| Regulation of biological process | 436 | (8.0%) | 11 | (1.7%) | 1,658 (7.3%) | ||
| Reproduction | 18 | ↓ | (0.3%) | 0 | ↓ | (0.0%) | 826 (3.6%) |
| Response to stimulus | 207 | ↓ | (3.8%) | 7 | (1.1%) | 1,402 (6.1%) | |
| Viral life cycle | 16 | ↑ | (0.3%) | 0 | (0.0%) | 1 (0.0%) | |
Listed are the numbers and percentages of assembled fire ant sequences and of D. melanogaster genes that match at least one of the second-level GO terms for molecular function, cellular component, or biological process. GO annotations for fire ant sequences were inferred electronically using two methods: blastx homology to GO-annotated proteins and Prosite protein domain scans. Statistically significant over- (↑) or underrepresentation (↓) of GO terms in fire ant relative to the Drosophila genome are indicated in bold (p < 10-8, Bonferroni-corrected hypergeometric test). *This number represents the sum of the numbers of occurences of GO terms below this level. †The 'cell part' and 'virion part' GO categories were excluded from analyses because they were redundant with the 'cell' and 'virion' categories, respectively.