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. 2007 Mar 30;3(3):e43. doi: 10.1371/journal.pgen.0030043

Figure 2. Assessment of Heat Shock Induction and Levels of Hsp90 Inhibition.

Figure 2

(A) Western blots to assess Hsp70 induction following overnight treatment of WT embryos initiated at 50% epiboly with increasing concentrations of radicicol or 17AAG. As positive control, Hsp70 was induced by incubating embryos at 37 °C for 30–60 min before harvesting.

(B) Western blots showing graded reduction in RAF1, an Hsp90 client-protein, in response to inhibition by radicicol and 17AAG.

(C) Western blots to assess Hsp90, Hsp70, and RAF1 levels in embryos injected with 6 ng of universal morpholino control (STD MO) or Hsp90 a+b morpholinos at the indicated individual amounts. α-tubulin was used as loading control.

*, Nonspecific band showing equal loading

HHS Vulnerability Disclosure