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. 1999 Oct 12;96(21):11952–11957. doi: 10.1073/pnas.96.21.11952

Figure 2.

Figure 2

RAG expression is down-regulated in the pro-B cell fraction of adult, but not fetal, M54 SCID mice. (A) Flow cytometric contour plots of sorted cells illustrate the different phenotypes of cell populations analyzed: pro-M/B (B220− CD43+IgM−) cells, pro-B (B220+CD43+IgM−), and pre-B (B220+CD43−IgM−) cells. The contour plots shown for pro-M/B and pro-B cells correspond to sorted cells from day 17 fetal liver (FL) of M54 SCID (s/s) mice; the contour plot for pre-B cells corresponds to sorted cells from bone marrow (BM) of M54 s/s adult mice. (B and C) Southern blots of RT-PCR-amplified RAG, μ, and λ5 transcripts in sorted cell populations of adult and day 17 fetal mice. Amplification of the β2M message served as an internal control for input cDNA. The first and second lanes under each bracket (from left to right) correspond to undiluted and 3-fold diluted input cDNA, respectively. The primers and probes used for amplification and hybridization of PCR products are described in Materials and Methods.