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Detection of LCMV-antigen in different spleens and kidneys of LCMV-immune B6 mice by immunohistological analysis. Spleens and kidneys were harvested 80 days after infection with 2 × 106 pfu LCMV-WE (C–E). Control sections from uninfected animals are shown (A and B). Frozen tissue sections were stained with VL-4, a rat anti-LCMV-NP monoclonal Ab (A–D). Paraffin sections were stained with a rabbit anti-LCMV serum [hyperimmune serum (His)] (E). (×400.)
