Figure 3.

Localization of two MARs flanking the core Eδ by using an in vitro MAR assay. DNA fragments released from pEδ3.8 by digestion with BamHI and SalI (A) or BamHI, XbaI, and SalI (B) were tested for in vitro binding to the nuclear matrix as in Fig. 1. The SalI site is contributed by the plasmid vector. (C) Alternatively, DNA fragments were released from pEδ0.4 by digestion with BamHI and SalI and were mixed with PCR-generated fragment l. Fragments were end-labeled and tested for nuclear matrix binding in vitro. (D) Schematic map of the Eδ region and the fragments tested in the in vitro MAR assay. B, BamHI; X, XbaI; D, DraI; Bg, BglII.