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. Author manuscript; available in PMC: 2008 Mar 6.
Published in final edited form as: FEBS Lett. 2007 Feb 7;581(5):949–954. doi: 10.1016/j.febslet.2007.01.087

Figure 4.

Figure 4

Induction of INSM1 expression in PANC-1 cells and ChIP analyses of CBP and acetyl-histone H3 and H4. (A) Relative RT-PCR analysis of gene expression in Ad-ngn3 transduced PANC-1 cells revealed induction of INSM1 expression as compared to the non-transduced or Ad-LacZ transduced cells. As a control, expression of Ad-ngn3 was performed. GAPDH was included as an internal control for the RT-PCR reaction. (B) ChIP analysis was performed with the Ad-LacZ or Ad-ngn3 transduced PANC-1 cells with an anti-CBP antibody. The CBP antibody could immunoprecipitate the same INSM1 promoter region bound by the ngn3 protein only in ngn3 transduced cells. (C) ChIP analysis was performed with the Ad-LacZ or Ad-ngn3 transduced PANC-1 cells with an anti-acetyl-H3 or anti-acetyl-H4 antibody. Anti-acetyl-H4 and to a lesser degree anti-acetyl H3 antibodies immunoprecipitated the INSM1 promoter only in the Ad-ngn3 transduced PANC-1 cells. Input DNA was included as a positive control (~2.5%) for PCR analysis. Each ChIP experiment was repeated to ensure its reproducibility.