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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: J Invest Dermatol. 2006 Oct 19;127(3):730–732. doi: 10.1038/sj.jid.5700607

Figure 1. LPS stimulates CRH in normal human adult epidermal keratinocytes.

Figure 1

Cells were incubated (a) with LPS (0–1,000 ng/ml) for 1 hour and (b) with LPS (1,000 ng/ml) for 1–24 hours. Cells were lysed, total RNA extracted, and real-time PCRs (n=3) performed for CRH. (c) Cells were incubated with LPS (0–1,000 ng/ml). After 24 hours, the supernatants were collected, concentrated on C18 SEP-COLUMNS, and CRH determined by ELISA (n=4). Data are presented as mean ± SEM. *P< 0.05 between untreated and LPS-treated cells.