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. Author manuscript; available in PMC: 2007 Mar 30.
Published in final edited form as: Mol Cell. 2005 Oct 28;20(2):225–236. doi: 10.1016/j.molcel.2005.08.026

Figure 2. Paf1 Complex Members Genetically Interact with NAB3 and NRD1.

Figure 2

(A) Vector (pRS425), the original 2μ library plasmid containing NAB3, and a NAB3 sub-clone (2μ NAB3) were transformed into wt (KY767), rtf1Δ (KY656), and rtf1-107 (KKY58) strains. Suppression of the Spt− phenotype was analyzed by spotting equivalent numbers of cells in 10-fold serial dilutions onto selective medium lacking histidine (5 days incubation).

(B) A plasmid shuffle experiment was performed by transforming pRS314, pRS314-NRD1, or pRS314-nrd1-5 into nrd1Δ RTF1(KKY129), nrd1Δ rtf1Δ (KKY128), or nrd1Δ cdc73Δ (KKY143) cells that contained pRS316-NRD1. Transformants were passaged on 5-FOA to select against the pRS316-NRD1 plasmid. Equivalent numbers of cells were spotted in 10-fold serial dilutions onto YPD (4 days incubation).