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. Author manuscript; available in PMC: 2007 Mar 30.
Published in final edited form as: Nature. 2006 Mar 23;440(7083):561–564. doi: 10.1038/nature04530

Figure 2. NGD is initiated by endonucleolytic cleavage of mRNA with various ribosomal stalls.

Figure 2

Steady-state cultures of xrn1Δ (a) and dom34Δxrn1Δ (b) strains with PGK1, PGK1-SL, PGK1-PK (pseudoknot), PGK1-RC (rare codons), PGK1-stop (stop codon) and PGK1-proline-stop (proline-stop codon) reporters were grown at low temperature (16 8C). Northern analysis of steady-state reporter mRNAs was performed with a probe specific for mRNA regions 3′ of the stall sites.