Table 1.
WNV antibody titers produced in mice to 80E and to NS1 proteins
| Group # | 80E dose (μg) | NS1 dose (μg) | ELISA titer to 80Ea | ELISA titer to NS1a | PRNT90 titerb |
|---|---|---|---|---|---|
| 1 | 0.3 | 1 | ~500 | 6450 (4120–10,100) | 640 |
| 2 | 1 | 1 | 10,420 (2410–45,100) | 4730 (2060–10,800) | 1280 |
| 3 | 0 | 0 | <250 | <250 | <10 |
a
Dilution of serum yielding 50% maximal absorbance in the ELISA as determined by a sigmoidal dose response, variable slope, mathematical model (GraphPad Prizm). Titers given are the GMT of individual mice (n = 5). Values in parenthesis: lower limit of 95% confidence interval (CI) to upper limit of 95% CI of GMT.
b
Highest dilution of serum (using 2-fold serial dilutions) yielding ≧90% reduction in plaques. Assays performed on pooled serum aliquots from each mouse within a group.