Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Dec;57(12):3405–3409. doi: 10.1128/aem.57.12.3405-3409.1991

Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40

Aidan Harrington 1, Colin Hill 1,*
PMCID: PMC183987  PMID: 16348595

Abstract

Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of α-acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39°C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A—production of high levels of α-acetolactic acid—is unaffected by the presence of pNP40.

Full text

PDF
3405

Images in this article

3406Image
on p.3406
3407Image
on p.3407

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Froseth B. R., Herman R. E., McKay L. L. Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3. Appl Environ Microbiol. 1988 Aug;54(8):2136–2139. doi: 10.1128/aem.54.8.2136-2139.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Froseth B. R., McKay L. L. Molecular characterization of the nisin resistance region of Lactococcus lactis subsp. lactis biovar diacetylactis DRC3. Appl Environ Microbiol. 1991 Mar;57(3):804–811. doi: 10.1128/aem.57.3.804-811.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gasson M. J. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol. 1983 Apr;154(1):1–9. doi: 10.1128/jb.154.1.1-9.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hill C., Massey I. J., Klaenhammer T. R. Rapid method to characterize lactococcal bacteriophage genomes. Appl Environ Microbiol. 1991 Jan;57(1):283–288. doi: 10.1128/aem.57.1.283-288.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hill C., Miller L. A., Klaenhammer T. R. Nucleotide sequence and distribution of the pTR2030 resistance determinant (hsp) which aborts bacteriophage infection in lactococci. Appl Environ Microbiol. 1990 Jul;56(7):2255–2258. doi: 10.1128/aem.56.7.2255-2258.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hill C., Pierce K., Klaenhammer T. R. The conjugative plasmid pTR2030 encodes two bacteriophage defense mechanisms in lactococci, restriction modification (R+/M+) and abortive infection (Hsp+). Appl Environ Microbiol. 1989 Sep;55(9):2416–2419. doi: 10.1128/aem.55.9.2416-2419.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jarvis A. W., Heap H. A., Limsowtin G. K. Resistance against Industrial Bacteriophages Conferred on Lactococci by Plasmid pAJ1106 and Related Plasmids. Appl Environ Microbiol. 1989 Jun;55(6):1537–1543. doi: 10.1128/aem.55.6.1537-1543.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKay L. L., Baldwin K. A. Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophage. Appl Environ Microbiol. 1984 Jan;47(1):68–74. doi: 10.1128/aem.47.1.68-74.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Baldwin K. A., Zottola E. A. Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972 Jun;23(6):1090–1096. doi: 10.1128/am.23.6.1090-1096.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanders M. E., Leonhard P. J., Sing W. D., Klaenhammer T. R. Conjugal strategy for construction of fast Acid-producing, bacteriophage-resistant lactic streptococci for use in dairy fermentations. Appl Environ Microbiol. 1986 Nov;52(5):1001–1007. doi: 10.1128/aem.52.5.1001-1007.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES