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. Author manuscript; available in PMC: 2007 Mar 30.
Published in final edited form as: Protein Expr Purif. 2006 Sep 12;52(1):50–58. doi: 10.1016/j.pep.2006.08.015

Figure 3.

Figure 3

(A) Collision-ionization-disassociation mass spectra of RApSAPLPGLSAPGR (residues 38–51 of TAT- HSP20, corresponding to residues 14–27 in wild-type human HSP20) observed in a tryptic digest of TAT-HSP20 isolated from bacterially-derived human TAT-HSP20 co-expressed with cPKG. MS/MS m/z 715.4, 2+. * indicates loss of H3PO4 (98 u) and $ indicates loss of H20 from the indicated fragment (18u). (B) MS/MS/MS, m/z spectrum 715.4 (2+) → 666.4(2+).