Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Summary of purification scheme used to purify phosphorylated TAT-HSP20
| Step 1 | Inclusion body isolation |
| Step 2 | Inclusion body solubilization in buffer containing 8M urea |
| Step 3 | Immobilized metal affinity chromatography |
| Step 4 | Overnight resin wash using 8M to 0M urea gradient |
| Step 5 | Elution using imidazole |
| Step 6 | Imidazole removal via stepwise dialysis against physiological saline solution |
