Table 1.
Proteins involved in ISC synthesis in Saccharomyces cerevisiae.
| Proteins | Protein Function | Type of evidence for Protein Function | Suggested Function In ISC Assembly | Type of evidence for suggested systemic role |
| Arh1 | Ferredoxin Reductase | Homology to known ferredoxin/adrenodoxin reductases | Reduces Yah1/Provides electrons for ISC assembly/transfer/repair | Homology, Physiological, Genetic |
| Yah1 | Ferredoxin | Homology to known ferredoxin/adrenodoxin | Provides electrons for ISC assembly/transfer/repair | Physiological, Genetic |
| Yfh1 | Frataxin | Homology, Biochemical; Structural | Stores/Provides Fe directly to ISC assembly and Heme synthesis | Physiological, Structural, Genetic |
| Grx5 | Glutaredoxin | Homology, Biochemical | Regulates glutathionylation state of protein cysteinyl residues | Physiological, Biochemical |
| Isa1 | Scaffold | As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins | Biochemical, Spectroscopic, Direct transfer observation | |
| Isa2 | Scaffold | As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins | Biochemical, Spectroscopic, Direct transfer observation | |
| Isu1 | Scaffold | As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins | Biochemical, Spectroscopic, Direct transfer observation | |
| Isu2 | Scaffold | As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins | Biochemical, Spectroscopic, Direct transfer observation | |
| Nfu1 | Scaffold | As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins | Biochemical, Spectroscopic | |
| Ssq1 | Hsp70 Chaperone | Homology, Biochemical, Genetic, Structural | Assists in proper folding of ISC biosynthetic proteins, namely Yfh1 and Isa-Isu proteins/Assists in maintaining ISC assembled in scaffold dimer for proper transfer | Biochemical, Structural |
| Jac1 | Hsp40 Co- chaperone | Homology, Biochemical, Genetic, Structural | Assists Ssq1 in interacting with Isu/Isa proteins | Biochemical, Genetic, Structural |
| Mge1 | Co- chaperone/Nucleotide exchange factor | Homology, Biochemical, Genetic, Structural | Assists Ssq1 | Biochemical, Genetic, Structural |
| Nfs1 | Cysteine Desulfurase | Homology, Biochemical | Provides sulphur for ISC assembly in scaffold dimers or in situ ISC assembly/repair | Physiological, Biochemical, Genetics |
| Atm1 | ABC transporter | Homology | Involved in ISC export for cytoplasm and nuclear proteins | Physiological, Genetic |
| Isd11 | unknown | Biochemical, Genetic | Fundamental for Nfs1 action | Physiological, Genetic |