Table 5.
Experimental values for the three in vitro flow chamber environments and the corresponding parameter values used for each of the three simulated experimental conditions
| In Vitro Values | ISWBC Values | |||||
| Experiment | Shear (dyn/cm2) | Substrate Molecule | Site density/Plating concentration | Rear Force | LIGAND | LIGAND Density |
| I. Neutrophil Rolling on P -Selectin | ||||||
| A. Pause Time | 0.5, 1.0, 2.0 | P-selectin | 9 sites/μm2 | 0.1–0.5 | PSELECTIN | 15 ± 5 |
| B. Distance-Time | 2 | P-selectin | 25 sites/μm2 | 0.5 | PSELECTIN | 25 ± 5 |
| C. Velocity-Time | 0.5 | P-selectin | 9 sites/μm2 | 0.1 | PSELECTIN | 15 ± 5 |
| II. T-Lymphocyte Rolling on VCAM-1 | ||||||
| Distance-Time | 0.73 – 7.3 | VCAM-1 | 15 μg/mLa | 0.15–1.6 | VCAM1 | 45 ± 5 |
| III. Monocyte Rolling on P-selectin/VCAM- 1/GRO-α | ||||||
| Rolling and Adhesion | 1 | VCAM-1 | 100 ng/mLa | 1 | VCAM1 | 13 ± 5 |
| P-Selectin | 10 μg/mLa | PSELECTIN | 25 ± 5 | |||
| GRO-α | 5 μg/mLa | GROA | 3 ± 2 | |||
The FLOW CHAMBER SURFACE dimensions were fixed at 100 × 60 SURFACE UNITS. Each SURFACE grid space maps to approximately 1 μm2 of effective flow chamber surface area. a Values listed in units of [μg/mL] are the concentrations of the solutions of receptors used to coat the parallel plate flow chamber surfaces and are not meant to represent the concentrations of receptors found on the parallel plate surface.