Figure 1.

Antigen-induced proliferation indices and disease status. Cells (2 × 10⁵) per well of fresh PBMC were cultured in round-bottomed, 96-well plates, as described in Methods, in medium alone or with 10 μg/ml gp120-depleted, inactivated HIV-1 (HIV), 10 μg/ml purified p24 antigen (p24), 10 μg/ml C. albicans (candida), or 20 μg/ml PHA (not shown). After 3 days (for PHA) or 6 days of incubation at 37°C, the cells were labeled with 1 μCi of ³[H]thymidine and harvested, and incorporated label was determined by scintillation counting. Geometric mean cpm were calculated from the triplicate wells with and without antigen. Results were calculated as an LSI, which is the geometric mean cpm of the cells plus antigen divided by the geometric mean cpm of the cells without antigen (medium alone). (Bars = SEM.)