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. Author manuscript; available in PMC: 2008 Jan 12.
Published in final edited form as: Mol Cell. 2007 Jan 12;25(1):151–159. doi: 10.1016/j.molcel.2006.12.008

Figure 2.

Figure 2

Mutation of K294 Affects Hsp90 Interaction with Client Proteins

(A) COS7 cells were co-transfected with ErbB2 (+) and indicated FLAG-Hsp90 constructs. Cells were lysed and FLAG-Hsp90 immunoprecipitates were separated by SDS-PAGE; ErbB2 binding was detected by immunoblotting.

(B) SkBr3 cells were transfected with indicated FLAG-Hsp90 constructs. Hsp90 was immunoprecipitated from cell lysates and ErbB2 binding was determined as in (A).

(C) NIH3T3 cells stably transfected with v-src were transfected with indicated FLAG-Hsp90 constructs. P60v-Src was immunoprecipitated.and associated FLAG-Hsp90 was detected by immunoblotting.

(D) SkBr3 cells were transfected with indicated FLAG-Hsp90 constructs. Endogenous mutant p53 was immunoprecipitated and associated FLAG-Hsp90 was detected as in (C).

(E) COS7 cells were co-transfected with HA-HIF-1αand indicated FLAG-Hsp90 constructs. HA-HIF-1αwas immunoprecipitated and associated FLAG-Hsp90 was detected as in (C).

(F) COS7 cells were co-transfected with androgen receptor and indicated FLAG-Hsp90 constructs. FLAG-Hsp90 was immunoprecipitated and androgen receptor was detected by immunoblotting.

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