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. Author manuscript; available in PMC: 2008 Jan 12.
Published in final edited form as: Mol Cell. 2007 Jan 12;25(1):151–159. doi: 10.1016/j.molcel.2006.12.008

Figure 3.

Figure 3

Mutation of K294 Affects Hsp90 Interaction with Co-chaperones

(A) COS7 cells were transfected with indicated FLAG-Hsp90 constructs. FLAG-Hsp90 was immunoprecipitated and associated p23 and p50cdc37 were detected by immunoblotting.

(B) COS7 cells were transfected as in (A). FKBP52 was immunoprecipitated and Hsp90 association was detected by immunoblotting.

(C) COS7 cells were co-transfected with FLAG-Aha1 and indicated HA-Hsp90 constructs. HA-Hsp90 was immunoprecipitated and associated FLAG-Aha1 was detected by immunoblotting.

(D) COS7 cells were transfected with indicated FLAG-Hsp90 constructs. FLAG-Hsp90 was immunoprecipitated and Hsp70 binding was detected by immunoblotting.

(E) COS7 cells were transfected with indicated FLAG-Hsp90 constructs. P60Hop was immunoprecipitated and FLAG-Hsp90 binding was detected by immunoblotting.

(F) COS7 cells were co-transfected with CHIP and indicated FLAG-Hsp90 constructs. FLAG-Hsp90 was immunoprecipitated and CHIP binding was detected by immunoblotting.

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