Figure 4.—

Stabilized Clb6 reverses loss of viability and replication control due to absence of Clb5. (A) Serial dilutions for MCM7-NLS clb5 GAL-CDC6ΔNT-HAm with CLB6Δ3P [gene replacement, encoding Clb6 stabilized due to mutation of phosphorylation sites (Jackson et al. 2006)] or CLB6-wt. (B) DNA content was measured by DNA flow cytometry analysis, as in Figure 2A. There is a minor peak at 4C DNA content in the CLB6Δ3P MCM7-NLS clb5 GAL-CDC6ΔNT-HAm strain. Microscopically, we did not detect significant clumping or division problems in this strain (data not shown). At present we do not know the explanation of this peak.