TABLE 2.
Summary of conclusions derived from genetic interactions and comparisons
| Strains and additional mutations (underlined) | Viability (serial dilution) | Rereplication (DNA flow cytometry analysis) |
|---|---|---|
| In CDC6ΔNT (no. 1) | Viable | No rereplication |
| clb5 from no. 2 | Clb5 stays alive | Clb5 blocks rereplication |
| MCM7-NLS from no. 4 | Mcm regulation has no effect | Mcm regulation has no effect |
| ORC6-ps,rxl from no. 7 | Orc regulation helps to stay alive | Orc regulation has no effect |
| In CDC6ΔNT clb5 (no. 2) | Reduced viability | Moderate rereplication |
| clb6 from no. 3 | Clb6 kills | Clb6 may drive rereplication |
| MCM7-NLS from no. 5 | Mcm regulation helps viability | Mcm regulation blocks rereplication |
| ORC6-ps,rxl from no. 8 | Orc regulation helps viability | Orc regulation may block rereplication |
| cdh1 from Figure 1 | Cdh1 kills | No data |
| In ORC6-ps,rxl CDC6ΔNT (no. 7) | Moderate reduced viability | No rereplication (2C DNA accumulation) |
| clb5 from no. 8 | Clb5 stays alive | Clb5 blocks rereplication |
| MCM7-NLS from no. 10 | Mcm regulation stays alive | Mcm regulation blocks rereplication |
| sic1 from Figure 1 | Sic1 regulation stays alive | No data |
| In ORC6-ps,rxl clb5 CDC6ΔNT (no. 8) | Lethal | Rereplication |
| clb6 from no. 9 | Clb6 kills | Clb6 drives rereplication |
| MCM7-NLS from tetrad | Mcm regulation stays alive | Mcm cannot test |
| cdh1 from Figure 3 | Cdh1 may kill | Cdh1 drives rereplication |
| In MCM7-NLS CDC6ΔNT (no. 4) | Viable | No rereplication |
| clb5 from no. 5 | Clb5 stays alive | Clb5 blocks rereplication |
| ORC6-ps,rxl from no. 10 | Orc regulation stays alive | Orc regulation blocks rereplication |
| In MCM7-NLS clb5 CDC6ΔNT (no. 5) | Lethal | Rereplication |
| clb6 From #6 | Clb6 helps killing | Clb6 drives rereplication |
| CLB6Δ3p from Figure 4 | Clb6 kills because of early proteolysis | Clb6 cannot block rereplication because of early proteolysis |
| cdh1 from Figure 3 | Cdh1 helps to kill | Cdh1 drives rereplication |
| In ORC6-ps,rxl MCM7-NLS CDC6ΔNT (no. 10) | Lethal | Rereplication |
| clb5 clb6 from no. 11 | Clb5,6 have little effect | Clb5,6 have little effect |
| In ORC6-ps,rxl MCM7-NLS (no. 10 on YEP-D) | Viable | No rereplication |
| clb5 from tetrad | Clb5 stays alive | Clb5 cannot be tested |
| In ORC6-ps,rxl MCM7-NLS clb5 | Lethal | Cannot test |
| clb6 from no. 11 on YEP-D | Clb6 kills | Clb6 cannot be tested |
| In clb5 clb6 GAL-CDC6ΔNT (no. 3) | Viable | No rereplication |
| MCM7-NLS from no. 6 | Mcm regulation may stay alive | Mcm regulation has no effect |
| ORC6-ps,rxl from no. 9 | Orc regulation has no effect | Orc regulation has no effect |
All of the mutant strains presented in this study are summarized. Strains that are underlined were compared to those with a single additional mutation. Results from tetrad analysis, serial dilution (Figures 1B, 3, and 4), and rereplication analysis (Figure 2A) are considered. “no.” refers to the strain from Figure 1B and Figure 2. “Orc regulation” refers to the ability to bind to and/or phosphorylate Orc6 and is based on the effects of the ORC6-ps,rxl mutation. “Mcm regulation” refers to the ability to regulate nuclear localization of the Mcm complex and is based on the effects of the MCM7-NLS mutation. See text.