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Analysis of Δtor1 Δtor2 double mutants. (A) Schematic of the opposite phenotypes of Δtor1 and Δtor2 mutations. (B) Terminal phenotype of Δtor1 Δtor2 cells expressing the nmt1-tor2+ construct (TA426) in the presence or absence of thiamine. (C) FACS analysis. Δtor2 or Δtor1 Δtor2 mutant cells expressing nmt1-tor2+ (TA313 and TA426, respectively) were grown to midlog phase and thiamine was added.
