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Localization and glycosylation of Spo1-6myc. Spheroplasts were prepared and stained with DAPI, Texas Red (for Spo1-6myc), and FITC (for GFP–HDEL) as described in materials and methods. The SPO1-6myc allele was expressed from the constitutive TPI1 promoter in vegetative cells (A, top) and during meiosis (A, bottom) from the native SPO1 promoter. (B) EndoH treatment of Spo1-6myc on a 7% PAGE gel.
