Figure 6.—

Suppression of spo1Δ by specific GPI proteins and the effect of plb3 on suppression. (A) Comparison of the expression profiles for the SPO1, CWP1, CWP2, and SPO19 genes, based on oligo microarray analysis (Primig et al. 2000). Values are shown as percentages of maximum induction. The arrow points to similar times of upregulation for the CWP1 and SPO19 transcripts (4-fold increase for CWP1 and >1500-fold for SPO19). (B) Complementation of the spo1Δ spore formation defect by high-copy plasmids carrying SPO1, CWP1, CWP2, and a chimeric construct expressing the CWP2 ORF from the CWP1 promoter. Asci were counted for 300 cells/sample in triplicate samples for each construct after 4 days of incubation on SPIII media at 30°. (C) The effect of plb3 on spore formation and complementation/suppression of spo1Δ . Ascus production in isogenic PLB3 (dark gray), spo1Δ (clear, no asci), and plb3Δ (light gray) diploids (bars 1–3) is shown. Complementation and/or suppression of spo1Δ assayed in isogenic spo1Δ PLB3 (dark gray) and spo1Δ plb3Δ (light gray) diploids carrying high-copy vectors with SPO1 (bars 4 and 5), CWP1 (bars 6 and 7), the CWP1/2 chimera (bars 8 and 9), or no insert (bars 10 and 11) is shown.