TABLE 1.
Yeast strains
| Strain | Relevant genotype | Source |
|---|---|---|
| REE2221 | MATahis2 leu1-12 spo1-1 ura3-1 | S. Klapholz (this lab) |
| REE945 | MATα ade2 can1 cyh2 his7 leu1-c lys2 met13-c spo1-1 trp5 tyr1 ura3-1 | S. Klapholz (this lab) |
| W303-1A; W303-1B | MATaade2 can1-100r his3-11,15 leu2-3,112 trp1-1 ura3-1; isogenic MATα | R. Rothstein (Columbia University) |
| GTY361; GTY362 | W303-1A; W303-1B spo1Δ∷KanMX6 | This work |
| GTY297; GTY298 | W303-1A; W303-1B spo14Δ∷KanMX6 | This work |
| GTY118; GTY119 | W303-1A; W303-1B spo19Δ∷KanMX6 | This work |
| GTY196; GTY197 | W303-1A; W303-1B spo23Δ∷KanM6 | This work |
| GTY274; GTY275 | W303-1A; W303-1B plb3Δ∷KanMX6 | This work |
| GTY286; GTY287 | W303-1A; W303-1B cwp1Δ∷KanMX6 | This work |
| GTY90; GTY91 | W303-1A; W303-1B spo1Δ∷HIS3 | Tevzadze et al. (2000) |
| GTY157; GTY158 | GTY90; GTY91 URA3:SPO1-6xMYC:ura3-1 | This work |
| GTY114; GTY115 | GTY90; GTY91 cwp1Δ∷URA3 | This work |
| GTY247; GTY248 | GTY90; GTY91 plb3Δ∷KanMX6 | This work |
| GTY321; GTY322 | GTY157; GTY158 SPO23-FLAG:KanMX6 | This work |
| GTY349; GTY350 | GTY90; GTY91 TRP1:HDEL-GFP:trp1-1 LEU2:TPI1p-SPO1-6xMYC:leu2-3,112 | This work |
| GTY357; GTY358 | GTY90; GTY91 TRP1:HDEL-GFP:trp1-1 LEU2:SPO1p-SPO1-6xMYC:leu2-3,112 | This work |
| yC66 | MATacan1-100 his3-11,15 leu1-12 lys2-1 trp1-1 tyr1-1 ura3-1 | Steber and Esposito (1995) |
| yC67 | MATα cyh2 his3-11,15 leu1-c met13-c trp1-1 tyr1-2 ura3-1 | Steber and Esposito (1995) |
| YAH67; YAH68 | yC66; yC67 ume4Δ∷HIS3 | Ann Helms (this lab) |
| yC105; yC106 | yC66; yC67 ume6Δ | Steber and Esposito (1995) |
| GTY52; GTY53 | yC66 ; yC67 spo14Δ∷URA3 | This work |
| GTY34; GTY35 | yC66; yC67 ime1Δ∷URA3 | This work |
| GTY54; GTY55 | GTY52; GTY53 spo1Δ∷TRP1 | This work |
| GTY61; GTY62 | yC66; C67 cwp1Δ∷URA3 | This work |
| GTY68; GTY69 | yC66; yC67 spo1Δ∷HIS3 | This work |
| GTY71; GTY72 | GTY68; GTY69 URA3:urs1-SPO1:ura3-1 | This work |
| GTY73; GTY74 | GTY68; GTY69 URA3:uash-SPO1-ura3-1 | This work |
| GTY112; GTY113 | GTY68; GTY69 cwp1Δ∷URA3 | This work |
| GTY203; GTY204 | yC66; yC67 spo1Δ∷KanMX6 | This work |
| GTY218; GTY219 | yC66; yC67 spo19Δ∷KanMX6 | This work |
| NSY72 | MATahis3-Δ200 lys2-801 sec14-1 ura3-52 | Nava Segev (University of Illinois, Chicago) |
| NSY73 | MATα ade2-1Δ 1 his3-Δ200 trp1-Δ sec14-1 ura3-52 | Nava Segev (University of Illinois, Chicago) |
| GTY200; GTY201 | NSY72; NSY73 spo1Δ∷KanMX6 | This work |
| B55 | MATaade2-101 his4-519 leu2-3,112 sec14-1 ts | V. Bankaitis (University of North Carolina) |
| GTY466 | B55 LEU2:YIplac-128T:leu2-3,112 | This work |
| GTY467 | B55 LEU2:TPI1p-SPO1:leu2-3,112 | This work |