Figure 3.—

Dmel\TIP60 and Dmel\ELP3 are each differentially expressed during Drosophila development. We performed real-time PCR analysis of Dmel\TIP60 and Dmel\ELP3 transcript levels using stage-specific D. melanogaster cDNAs (12- to 24-hr staged embryos; first, second, and third instar larvae; pupae; adult flies) prepared by RT priming of DNase-treated RNA with random hexamers and PCR primer sets amplifying 200-bp regions specific for each dHAT. The histogram depicts RNA copy number (mean + SD) in logarithmic scale of at least three independent experiments for both Dmel\TIP60 and Dmel\ELP3 in each stage of development. SYBR-green kit and Opticon2 system (MJ Research) were used for real-time detection and data analysis. All data shown are corrected for −RT background.