Figure 6.—

Expression of Dmel\TIP60/RNAi in three independent fly lines reduces endogenous Dmel\TIP60 levels. (A) Progeny resulting from a cross between homozygous Dmel\TIP60/RNAi (independent lines Dmel\TIP60/RNAi/A, -B, and -C) or Dmel\TIP60/control (independent lines Dmel\TIP60/control/A, -B, and -C) and ubiquitous GAL4 line 337 were allowed to develop to the third instar larval stage. RNA was isolated from three third instar larvae progeny and subjected to semiquantitative RT–PCR analysis. cDNAs were obtained from equal amounts of RNA for each sample using RT priming with random hexamers. PCR primer sets were specific for either Dmel\TIP60 that did not amplify Dmel\TIP60 RNAi target sequences or RP49. All RT–PCR experiments included negative (−RT) controls for both Dmel\TIP60 and RP49, which showed no background in all samples tested (data not shown). All experiments were repeated at least twice with consistent results. This figure shows RT–PCR analysis of one representative experiment.