TABLE 1.
Ubiquitous expression of Dmel\TIP60/RNAi in three independent fly lines results in total lethality of developing flies
| Fly linesa | GAL4+(y; Cy+)b | GAL4−(y+; Cy)b |
|---|---|---|
| Dmel\TIP60/RNAi/A | 0 ± 0 | 49 ± 11 |
| Dmel\TIP60/RNAi/B | 0 ± 0 | 53 ± 12 |
| Dmel\TIP60/RNAi/C | 0 ± 0 | 57 ± 14 |
| Dmel\TIP60/control/A | 67 ± 16 | 63 ± 7 |
| Dmel\TIP60/control/B | 57 ± 0 | 59 ± 8 |
| Dmel\TIP60/control/C | 69 ± 3 | 67 ± 12 |
Three flies homozygous for either Dmel\TIP60/RNAi or Dmel\TIP60/control P-element insertions were mated to three flies homozygous for the actin GAL4 driver line Act5c-GAL4: (Dmel\TIP60/RNAi or control × P{Act5c-GAL4}/CyO,y+). For Dmel\TIP60/RNAi lines, the P-element insertion is located on the X chromosome for line A and on the second chromosome for lines B and C. For Dmel\TIP60/control lines, the P-element insertion is located on the second chromosome for line A and on the X chromosome for lines B and C.
Adult progeny were counted over a 10-day period and scored for either GAL4+(y;Cy+) or GAL4−(y+;Cy) phenotypes. All three Dmel\TIP60/RNAi lines strongly reduced viability to 0% that of the Dmel\TIP60/control lines. Lethality for the majority of flies occurred during pupal development. The results are reported as mean ±SD (n = 3).