Figure 3.—

Promoter constructs of Cyp6g1 from Canton-S, y; cn bw sp, and Hikone-R (containing the Accord LTR) expressing nuclear-localized GFP replicate the expression pattern seen by in situ hybridization. (A and B) Expression of GFP in the midgut (MG) and Malpighian tubules (MT) is detected using the Cyp6g1 promoter of Canton-S in third instar larvae. (C and D) Expression of GFP in the midgut (MG) and Malpighian tubules (MT) is detected using the Cyp6g1 promoter of y;cn bw sp. Faint expression of GFP in the fat body was detected only in wandering third instar larvae but not feeding third instar larvae. (E and F) Expression of GFP in the MG, MT, gastric caecae (GC), and the fat body (FB) is detected throughout the third instar larval stage when GFP is driven by the promoter of Hikone-R (containing the Accord LTR).