Figure 1.—

Derepression of nucleoporin-mediated transcription requires the Snf1 kinase complex. Wild type (WT), Δsnf1, Δgal83, and Δsnf4 strains containing a chromosomally integrated lexA-driven reporter gene were transformed with nucleoporin-lexA fusion genes as described previously and assayed for β-galactosidase activity after growth in either the presence (open bars, repression) or the absence (shaded bars, derepression) of glucose. Each of the nucleoporin-lexA fusion genes tested in this assay was expressed at normal levels, i.e., was driven by its native promoter. LexA fusion genes are (A) NUP84, (B) NUP145C, (C) NUP120, (D) NUP145N, (E) SEC13, and (F) NUP133. Error bars represent the standard deviation of four independent determinations. Reporter expression driven by control proteins, which included Ste12-lexA and Gcn4-lexA chimeras, was similar in the presence or absence of Snf1 kinase subunits.