TABLE 1.
Nucleoporin activation is repressed by glucose
| lexA fusiona | R (glucose) | D (no glucose) | D/R ratiob |
|---|---|---|---|
| NUP84 | 2303c | 5185c | 2.3 |
| NUP145C | 799 | 3403 | 4.3 |
| NUP120 | 191 | 766 | 4.0 |
| NUP145N | 65 | 521 | 8.0 |
| SEC13 | 17 | 87 | 5.1 |
| NUP133 | 7 | 57 | 8.1 |
| STE12 | 124 | 133 | 1.1 |
| lexA alone | <0.1 | <0.1 | — |
a
C-terminal fusion to the lexA DNA-binding domain. The classically described STE12 activator and lexA DNA-binding domain alone are shown as controls.
b
Ratio of derepressed to repressed reporter gene expression.
c
lexA-driven β-galactosidase activity. Four independent determinations of β-galactosidase activity (units per milligram of total protein) were averaged; standard error of the mean for each value was <10%.