Figure 1.—

Lethality caused by transposase-induced DSBs. (A) Lethality using chromosome 2 transposase and a P{w^a} element. (B) Lethality using chromosome 3 transposase and three P elements (P{w^a} and two at the sn locus). DSBs occur in the somatic tissue and germline of the male progeny of the indicated zygotic genotype that have both the P{w^a} element and transposase (A) or sn^w and P{w^a} elements and transposase (B; see materials and methods for details). The percentage expected is determined by the number of males of the indicated genotype with transposase relative to brothers without transposase. Bars represent means; error bars indicate standard deviation from three independent experiments (wild-type, mei-41, and grp lok mutants) or range from two experiments (other genotypes).