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cAMP response to hCG stimulation in granulosa cells. Granulosa cells isolated from antral follicles of the PMSG-treated mice were cultured in the absence (basal) or presence of 100 ng/ml hCG. After 1-h incubation, the intracellular cAMP accumulation was measured by RIA. Data are the mean ± SEM (n = 4 independent experiments each carried out in triplicate). The difference between PDE4D+/− and PDE4D−/− mice in response to hCG is significant (P < 0.001 determined by paired t test, *).
